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Universal immuno-PCR for ultra-sensitive target protein detection.

作者信息

Zhou H, Fisher R J, Papas T S

机构信息

Laboratory of Cellular Biochemistry, PRI/DynCorp, Frederick, MD 21702.

出版信息

Nucleic Acids Res. 1993 Dec 25;21(25):6038-9. doi: 10.1093/nar/21.25.6038.

DOI:10.1093/nar/21.25.6038
PMID:8290366
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC310491/
Abstract
摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d1c7/310491/4946d6b05c12/nar00074-0244-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d1c7/310491/4946d6b05c12/nar00074-0244-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d1c7/310491/4946d6b05c12/nar00074-0244-a.jpg

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Response.反应
Science. 1993 Apr 30;260(5108):699. doi: 10.1126/science.260.5108.699.
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Immuno-PCR with a commercially available avidin system.使用市售抗生物素蛋白系统的免疫聚合酶链反应。
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Simple objective detection of human lyme disease infection using immuno-PCR and a single recombinant hybrid antigen.使用免疫聚合酶链反应和单一重组杂交抗原来简单客观地检测人类莱姆病感染。
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Development of a highly sensitive immuno-PCR assay for the measurement of α-galactosidase A protein levels in serum and plasma.开发一种高度敏感的免疫-PCR 测定法，用于测量血清和血浆中的α-半乳糖苷酶 A 蛋白水平。
PLoS One. 2013 Nov 13;8(11):e78588. doi: 10.1371/journal.pone.0078588. eCollection 2013.
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Nucleic acids for ultra-sensitive protein detection.用于超高灵敏度蛋白质检测的核酸。
Sensors (Basel). 2013 Jan 21;13(1):1353-84. doi: 10.3390/s130101353.
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Detection of respiratory syncytial virus using nanoparticle amplified immuno-polymerase chain reaction.利用纳米颗粒扩增免疫聚合酶链反应检测呼吸道合胞病毒。
Anal Biochem. 2011 Mar 1;410(1):141-8. doi: 10.1016/j.ab.2010.11.033. Epub 2010 Nov 25.
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Microsphere-based rolling circle amplification microarray for the detection of DNA and proteins in a single assay.基于微球的滚环扩增微阵列用于在单一检测中同时检测DNA和蛋白质。
Anal Chem. 2009 Jul 15;81(14):5777-82. doi: 10.1021/ac900694y.
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Combination of DNA-directed immobilization and immuno-PCR: very sensitive antigen detection by means of self-assembled DNA-protein conjugates.DNA 定向固定与免疫聚合酶链反应相结合：借助自组装 DNA-蛋白质缀合物进行超灵敏抗原检测。
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Science. 1985 Dec 20;230(4732):1350-4. doi: 10.1126/science.2999980.
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Isoforms of the human ets-1 protein: generation by alternative splicing and differential phosphorylation.人类ets-1蛋白的异构体：通过可变剪接和差异磷酸化产生
Oncogene. 1990 May;5(5):675-81.
5
Reciprocal expression of human ETS1 and ETS2 genes during T-cell activation: regulatory role for the protooncogene ETS1.人类ETS1和ETS2基因在T细胞激活过程中的相互表达：原癌基因ETS1的调节作用。
Proc Natl Acad Sci U S A. 1990 May;87(10):3723-7. doi: 10.1073/pnas.87.10.3723.
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By-passing immunization. Human antibodies from V-gene libraries displayed on phage.绕过免疫接种。噬菌体展示的来自V基因文库的人抗体。
J Mol Biol. 1991 Dec 5;222(3):581-97. doi: 10.1016/0022-2836(91)90498-u.
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Immuno-PCR: very sensitive antigen detection by means of specific antibody-DNA conjugates.免疫聚合酶链反应：借助特异性抗体 - DNA 偶联物进行超灵敏抗原检测。
Science. 1992 Oct 2;258(5079):120-2. doi: 10.1126/science.1439758.
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Competitive PCR.
Nature. 1992 Oct 8;359(6395):557-8. doi: 10.1038/359557a0.