Bhat N K, Thompson C B, Lindsten T, June C H, Fujiwara S, Koizumi S, Fisher R J, Papas T S
Laboratory of Molecular Oncology, National Cancer Institute, Frederick, MD 21701-1013.
Proc Natl Acad Sci U S A. 1990 May;87(10):3723-7. doi: 10.1073/pnas.87.10.3723.
The expression of the protooncogenes ETS1 and ETS2 has been studied in purified human T cells activated either by cross-linking of the T-cell receptor-CD3 complex on their cell surface or by direct stimulation with phorbol esters and ionomycin. Our results show that resting T cells express high levels of ETS1 mRNA and protein, while expression of ETS2 is undetectable. Upon T-cell activation, ETS2 mRNA and proteins are induced, while ETS1 gene expression decreases to very low levels. Late after stimulation, ETS1 mRNA is reinduced and maintained at a high level, while ETS2 gene expression decreases to undetectable levels. Therefore, it appears that in human T cells, ETS2 gene products are associated with cellular activation and proliferation, while ETS1 gene products are preferentially expressed in a quiescent state.
在纯化的人T细胞中，研究了原癌基因ETS1和ETS2的表达情况。这些T细胞通过其细胞表面的T细胞受体-CD3复合物交联或用佛波酯和离子霉素直接刺激而被激活。我们的结果表明，静息T细胞表达高水平的ETS1 mRNA和蛋白质，而ETS2的表达则无法检测到。T细胞激活后，ETS2 mRNA和蛋白质被诱导，而ETS1基因表达降至非常低的水平。刺激后期，ETS1 mRNA被重新诱导并维持在高水平，而ETS2基因表达降至无法检测的水平。因此，在人T细胞中，ETS2基因产物似乎与细胞激活和增殖相关，而ETS1基因产物则优先在静止状态下表达。
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