Pasotti D, Mazzone A, Fossati G, Mazzucchelli I, Pistone M C, Montagna M, Parachini N, Labbate G, Corti C, Ricevuti G
Istituto Superiore di Sanità, Roma.
Recenti Prog Med. 1993 Nov;84(11):742-9.
The aim of the present study was to evaluate some functions of neutrophil granulocytes (PMNs), such as aggregation, superoxide production, chemotaxis and adhesion molecules involved in these processes, in 22 patients suffering from Myelodysplastic Syndrome (MDS), to clarify if granulocytes alterations described in this syndrome is really correlated with the expression of surface membrane integrins. Several patients suffering from MDS present granulocytopenia and/or absolute monocytoses; neutrophil granulocytes can have typical nuclear and cytoplasmatic alterations. These granulocytic anomalies are valuable in about 90% of patients suffering from MDS. The granulocytes showed a significant deficit in chemotaxis stimulated by serum activated with E. Coli, casein and formyl-methionyl-leucylphenylalanine (fMLP) (p < 0.01) and in superoxide production stimulated by phorbol-myristate-acetate (PMA). We also studied the role of membrane integrin CD11/CD18 using specific monoclonal antibodies (MoAb). The cytofluorimetric analysis demonstrated a significant inhibition in expression of CD11b/CD18 receptors in patients suffering from MDS (p < 0.001), while the expression of CD11a/CD18 and CD11c/CD18 receptors was normal. In conclusion we found specific alterations in PMNs functions in MDS and a correlation of these anomalies with membrane integrins of PMNs is therefore possible.
本研究的目的是评估22例骨髓增生异常综合征（MDS）患者中性粒细胞的某些功能，如聚集、超氧化物生成、趋化性以及参与这些过程的黏附分子，以阐明该综合征中所描述的粒细胞改变是否真的与表面膜整合素的表达相关。几位MDS患者存在粒细胞减少和/或绝对单核细胞增多；中性粒细胞可出现典型的核和细胞质改变。这些粒细胞异常在约90%的MDS患者中具有诊断价值。粒细胞在受到大肠杆菌、酪蛋白和甲酰甲硫氨酰亮氨酰苯丙氨酸（fMLP）激活的血清刺激时趋化性显著降低（p < 0.01），在受到佛波酯 - 肉豆蔻酸酯 - 乙酸酯（PMA）刺激时超氧化物生成显著降低。我们还使用特异性单克隆抗体（MoAb）研究了膜整合素CD11/CD18的作用。细胞荧光分析表明，MDS患者中CD11b/CD18受体的表达受到显著抑制（p < 0.001），而CD11a/CD18和CD11c/CD18受体的表达正常。总之，我们发现MDS患者中性粒细胞功能存在特异性改变，因此这些异常与中性粒细胞的膜整合素之间可能存在相关性。
