骨髓增生异常综合征中的CD11/CD18粒细胞黏附分子

The CD11/CD18 granulocyte adhesion molecules in myelodysplastic syndromes.

作者信息

Mazzone A, Ricevuti G, Pasotti D, Fossati G, Mazzucchelli I, Cavigliano P, Notario A

机构信息

Department of Internal Medicine and Therapeutics, University of Pavia, IRCCS San Matteo Hospital, Italy.

出版信息

Br J Haematol. 1993 Feb;83(2):245-52. doi: 10.1111/j.1365-2141.1993.tb08279.x.

DOI:10.1111/j.1365-2141.1993.tb08279.x

PMID:8096150

Abstract

We have evaluated the function of granulocytes in 14 patients suffering from myelodysplastic syndrome (MDS). We also evaluated the functional and immunochemical activities of five monoclonal antibodies (MoAbs) reactive with the CD11/CD18 leucocyte adhesion molecules of granulocytes. Granulocytes showed a decrease in chemotaxis (P < 0.001) and in aggregation (P < 0.01) using various agents as a stimulus. Cytofluorimetric and immunoenzymatic assays with alkaline phosphatase (APAAP) analysis showed decreased expression of the CD11b/CD18 receptor detected by OKM1 (P < 0.001). Despite LFA-1 and-CD11a/CD18 was expressed in normal amounts. The studies of upregulation of granulocytes CD11b/CD18 and image analysis of immunochemical preparation (APAAP) demonstrated decreased expression of CD11b/CD18 in granulocytes from MDS compared to controls (P < 0.001). We conclude that granulocyte dysfunction in MDS may be correlated with decreased expression of surface CD11b/CD18 leucocyte adhesion molecules or their structural modification.

摘要

我们评估了14例骨髓增生异常综合征（MDS）患者中性粒细胞的功能。我们还评估了五种与粒细胞CD11/CD18白细胞粘附分子反应的单克隆抗体（MoAbs）的功能和免疫化学活性。使用各种试剂作为刺激物时，中性粒细胞的趋化性（P < 0.001）和聚集性（P < 0.01）均降低。通过碱性磷酸酶（APAAP）分析进行的细胞荧光分析和免疫酶分析显示，OKM1检测到的CD11b/CD18受体表达降低（P < 0.001）。尽管淋巴细胞功能相关抗原-1（LFA-1）和CD11a/CD18表达量正常。对MDS患者中性粒细胞CD11b/CD18上调的研究以及免疫化学制剂（APAAP）的图像分析表明，与对照组相比，MDS患者中性粒细胞中CD11b/CD18的表达降低（P < 0.001）。我们得出结论，MDS患者中性粒细胞功能障碍可能与表面CD11b/CD18白细胞粘附分子表达降低或其结构改变有关。