奥斯特他线虫和牛古柏线虫第三期感染性幼虫体蛋白的SDS-PAGE图谱。

SDS-PAGE profiles of somatic proteins from third-stage infective larvae of Ostertagia ostertagi and Cooperia oncophora.

作者信息

Baker D G, Gershwin L J

机构信息

Department of Veterinary Microbiology and Immunology, School of Veterinary Medicine, University of California, Davis 95616.

出版信息

Vet Parasitol. 1993 Oct;50(1-2):157-60. doi: 10.1016/0304-4017(93)90017-h.

DOI:10.1016/0304-4017(93)90017-h

PMID:8291192

Abstract

Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) profiles of somatic proteins from third-stage infective larvae of Ostertagia ostertagi and Cooperia oncophora were evaluated using laser densitometry. Several protein bands were present from both parasite preparations. A few bands from each parasite appeared to be unique. Purification of these proteins for use in serologic monitoring of cattle naturally infected with O. ostertagi and C. oncophora should allow circumvention of cross-reactivity between the two genera, which has been reported by others.

摘要

使用激光密度测定法评估了奥氏奥斯特他线虫和牛古柏线虫第三期感染性幼虫体蛋白的十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）图谱。两种寄生虫制剂均出现了几条蛋白带。每种寄生虫的几条带似乎是独特的。纯化这些蛋白质用于对自然感染奥氏奥斯特他线虫和牛古柏线虫的牛进行血清学监测，应可避免其他研究人员报道的这两个属之间的交叉反应。