Leech D, Rechnitz G A
Hawaii Biosensor Laboratory, Department of Chemistry, University of Hawaii, Honolulu 96822.
Anal Chem. 1993 Nov 15;65(22):3262-6. doi: 10.1021/ac00070a016.
In this report we demonstrate the first analytical application of biomagnetic field detection at nerve fibers for biosensing purposes. A ferrite core toroid surrounding the nerve, coupled to a low-noise, low-input-impedance amplifier, is used to inductively detect the compound action current (CAC) in crayfish giant axons upon stimulation of nerve firing. Detection of the local anesthetic lidocaine, which blocks neuronal conduction by binding in the ion channel of the voltage-gated sodium channel receptor, is achieved by monitoring the disappearance of the CAC. The application of this novel detection principle to the screening of neurotoxic and neuromodulatory drugs and natural product extracts is proposed.
在本报告中，我们展示了用于生物传感目的的神经纤维生物磁场检测的首次分析应用。围绕神经的铁氧体磁芯环形线圈与低噪声、低输入阻抗放大器相连，用于在刺激神经放电时感应检测小龙虾巨轴突中的复合动作电流（CAC）。通过监测CAC的消失来实现对局部麻醉药利多卡因的检测，利多卡因通过结合电压门控钠通道受体的离子通道来阻断神经元传导。本文提出了将这种新型检测原理应用于神经毒性和神经调节药物以及天然产物提取物筛选的方法。
