Coon D R, Babb C W, Rechnitz G A
Hawaii Biosensor Laboratory, Department of Chemistry, University of Hawaii, Honolulu 96822.
Anal Chem. 1994 Oct 1;66(19):3193-7. doi: 10.1021/ac00091a031.
We report the generation of action potentials in crayfish neurons by magnetic pulses for analytical purposes. A copper wire toroid, containing a ferrite core, was placed around the nerve bundle, and square wave current pulses were sent through the wire to generate a magnetic field. The magnetic field generated an action potential in the neuron that was detected downfield by a pickup microelectrode. The biosensor was used to detect local anesthetics by monitoring the time necessary for complete blockage of the action potential. Techniques for improving the efficiency and lifetimes of neural biosensors are discussed.
为了进行分析，我们报告了通过磁脉冲在小龙虾神经元中产生动作电位的情况。一个包含铁氧体磁芯的铜丝环形线圈被放置在神经束周围，方波电流脉冲通过该线圈发送以产生磁场。该磁场在神经元中产生一个动作电位，该动作电位由一个拾取微电极在下游检测到。生物传感器通过监测动作电位完全阻断所需的时间来检测局部麻醉剂。文中还讨论了提高神经生物传感器效率和寿命的技术。
