Lierl M B, Riordan M M, Fischer T J
Division of Allergy/Immunology, Children's Hospital Medical Center, Cincinnati, Ohio 45229-2899.
Ann Allergy. 1994 Jan;72(1):45-50.
Airborne insect antigens can represent significant outdoor aeroallergens. In this pilot study, allergic asthmatic children were screened by RAST for IgE directed against common outdoor insects. Insects studied included ant, cricket, grasshopper, housefly, and moth. Spiders were also tested. Three different extraction procedures were compared to determine which method(s) best preserves the allergenic proteins. RAST discs were made for each insect extract and sera from allergic, asthmatic and nonallergic, nonasthmatic children were tested. The allergic asthmatic sera had a higher percentage of isotope binding than the nonallergic nonasthmatic sera for all insects tested. The number of subjects with a Phadebas RAST score of 2 or greater was significantly higher in the allergic asthmatic group for moth (16 of 37, 43%), housefly (7 of 30, 23%), cricket (8 of 41, 19%), and grasshopper (7 of 36, 19%). Only one of the 25 control sera had a score of 2 to moth; none of the control sera reacted to any of the other insects. Most positive sera tested showed cross-reactivity between insects. A few sera, despite showing positive RAST results to two different extracts, showed no cross-reactivity between the two extracts, suggesting that these sera contained species-specific IgE. These results indicate that a significant proportion of allergic asthmatic children have IgE that binds to extracts of moth, cricket, grasshopper, and housefly. Further studies are needed to determine whether these insect aeroallergens can produce clinically significant symptoms in sensitized atopic individuals.
空气中的昆虫抗原可能是重要的室外空气过敏原。在这项初步研究中，通过放射变应原吸附试验（RAST）对过敏性哮喘儿童进行筛查，以检测针对常见室外昆虫的免疫球蛋白E（IgE）。所研究的昆虫包括蚂蚁、蟋蟀、蚱蜢、家蝇和飞蛾。还对蜘蛛进行了检测。比较了三种不同的提取程序，以确定哪种方法能最好地保存过敏原蛋白。为每种昆虫提取物制作了RAST盘，并检测了过敏性哮喘儿童和非过敏性非哮喘儿童的血清。对于所有测试的昆虫，过敏性哮喘血清的同位素结合百分比均高于非过敏性非哮喘血清。在过敏性哮喘组中，法德巴斯RAST评分为2或更高的受试者数量，在飞蛾（37例中的16例，43%）、家蝇（30例中的7例，23%）、蟋蟀（41例中的8例，19%）和蚱蜢（36例中的7例，19%）中显著更高。25份对照血清中只有1份对飞蛾的评分为2；对照血清对其他任何昆虫均无反应。大多数检测的阳性血清显示昆虫之间存在交叉反应。少数血清尽管对两种不同提取物的RAST结果呈阳性，但在两种提取物之间未显示交叉反应，这表明这些血清含有物种特异性IgE。这些结果表明，相当一部分过敏性哮喘儿童的IgE与飞蛾、蟋蟀、蚱蜢和家蝇的提取物结合。需要进一步研究以确定这些昆虫空气过敏原是否能在致敏的特应性个体中产生具有临床意义的症状。
