利用人-小鼠体细胞杂种将编码色氨酰-tRNA合成酶的人类基因定位于14号染色体。

Assignment of a human gene for tryptophanyl-tRNA synthetase to chromosome 14 using human-mouse somatic cell hybrids.

作者信息

Shimizu N, Kucherlapati R S, Ruddle F H

出版信息

Somatic Cell Genet. 1976 Jul;2(4):345-57. doi: 10.1007/BF01538839.

DOI:10.1007/BF01538839

Abstract

Human tryptophanyl-tRNA SYNTHETASE (Trp-RS, EC 6.1.1.2) can be separated from its mouse counterpart by Cellogel electrophoresis. Analysis of the presence or absence of human Trp-RS and other human enzyme markers in eleven independently dervied cell lines of human-mouse somatic cell hybrids revealed that the expression of Trp-RS is correlated with the expression of human nucleoside phosphorylase (NP, EC 2.4.2.1). The syntenic relationship between Trp-RS and NP permits the assignment of the structural gene for Trp-RS to human chromosome 14. Karyotype and isozyme analysis of these hybrid clones rules out other linkage assignments.

摘要

人色氨酰 - tRNA合成酶（Trp - RS，EC 6.1.1.2）可通过Cellogel电泳与小鼠对应物分离。对11个人 - 鼠体细胞杂交的独立衍生细胞系中人Trp - RS和其他人酶标志物的存在与否进行分析，结果表明Trp - RS的表达与人类核苷磷酸化酶（NP，EC 2.4.2.1）的表达相关。Trp - RS和NP之间的同线关系使得可以将Trp - RS的结构基因定位到人类14号染色体上。这些杂交克隆的核型和同工酶分析排除了其他连锁定位。

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引用本文的文献

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