De Lucia F, Faraone-Mennella M R, D'Erme M, Quesada P, Caiafa P, Farina B
Dipartimento di Chimica Organica e Biologica, Universit Federico II, Napoli, Italy.
Biochem Biophys Res Commun. 1994 Jan 14;198(1):32-9. doi: 10.1006/bbrc.1994.1005.
Due the likely role of H1 histone variants in inducing the formation of folded DNA filaments with different stabilities, the condensing capacity of the testis-specific H1t versus the somatic variants was tested. Circular dichroism analyses of rat testis H1-depleted oligonucleosomes (5-2kbp) revealed that H1t, which appears in germ cells during the meiotic prophase of mammalian spermatogenesis, exerts the lowest condensing effect as compared to the other variants. The distribution of H1 subtypes among different chromatin fractions was also investigated and gave evidence that H1t is more abundant in chromatin regions which are more sensitive to DNAase I digestion.
由于H1组蛋白变体在诱导形成具有不同稳定性的折叠DNA细丝中可能发挥的作用，对睾丸特异性H1t与体细胞变体的凝聚能力进行了测试。对大鼠睾丸H1缺失的寡核小体（5-2kbp）进行的圆二色性分析表明，在哺乳动物精子发生减数分裂前期出现在生殖细胞中的H1t，与其他变体相比，具有最低的凝聚效应。还研究了不同染色质组分中H1亚型的分布，结果表明H1t在对DNA酶I消化更敏感的染色质区域中更为丰富。
