Turovets I, Lewis A, Palanker D, Gilo H, Vilenz A, Broder J C, Lewis S
Hebrew University of Jerusalem, Israel.
Biotechniques. 1993 Dec;15(6):1022-4, 1026, 1028-9.
Poration of millions of cells with single laser pulses of an argon fluoride excimer laser is developed and demonstrated on plant cells. The essence of the technique is to use the large beam size (10 x 25 mm) of the excimer laser and to split it using an appropriate mask into millions of micron- or submicron-sized beams of 193-nm radiation with enough energy to perforate cell membranes and walls. The extremely small depth of penetration of the 193-nm radiation in biological tissue (< 1 microns) is used to great advantage in this technique to provide for a gentle method that does not lead to cell death. This is the first laser-based method that has enough throughput to make it viable for biotechnological applications. Of considerable significance is that the method is applicable not only in the case of the single cells in suspension, but also for cells growing on the leaves of living plants.
利用氟化氩准分子激光的单个激光脉冲对数百万个细胞进行穿孔的技术已被开发出来，并在植物细胞上得到了验证。该技术的核心是利用准分子激光的大光斑尺寸（10×25毫米），并使用合适的掩膜将其分割成数百万个微米或亚微米尺寸的193纳米辐射光束，这些光束具有足够的能量来穿透细胞膜和细胞壁。193纳米辐射在生物组织中的穿透深度极浅（<1微米），在这项技术中得到了充分利用，从而提供了一种不会导致细胞死亡的温和方法。这是第一种基于激光的方法，其通量足以使其在生物技术应用中可行。具有重要意义的是，该方法不仅适用于悬浮状态的单个细胞，也适用于生长在活植物叶片上的细胞。
