Kolesnikov N N, Slobodianiuk S Ia, Kikhmadze I I
Ontogenez. 1976;7(6):547-57.
The proteins of the salivary gland secretion at the moment of puparium formation and the protein composition of the salivary glands, fat-body and hemolymph during the III larval instar and 12 hours prepupal period were investigated by means of electrophoresis in polyacrilamide gel, in the presence of urea. Both the qualitative and quantitative differences in the protein composition of the salivary glands were found which appeared to be due to the change of functions of the salivary glands in the process of development. 69 protein fractions were isolated in the salivary gland secretion, 40 of them being PAS-positive. A correlation was noted between the appearance of the first PAS-positive fractions at the stage of 95-100 hrs (from the moment of egg laying) and the cytological demonstration of PAS-positive secretion granules. New glycoproteins appeared in the salivary glands in prepupae (2 hrs after the puparium formation) and, later, in the hemolymph. A complicated pattern of the secretion formation in the salivary glands of the III instar larvae and possible change of its composition during metamorphosis are discussed.
采用在尿素存在下的聚丙烯酰胺凝胶电泳法，对蛹形成时刻唾液腺分泌物的蛋白质以及三龄幼虫期和化蛹前12小时唾液腺、脂肪体和血淋巴的蛋白质组成进行了研究。发现唾液腺蛋白质组成在定性和定量上均存在差异，这似乎是由于唾液腺在发育过程中功能的变化所致。在唾液腺分泌物中分离出69个蛋白质组分，其中40个为PAS阳性。在95 - 100小时（从产卵时刻起）阶段首次出现PAS阳性组分与PAS阳性分泌颗粒的细胞学表现之间存在相关性。化蛹前期（蛹形成后2小时）唾液腺中出现了新的糖蛋白，随后血淋巴中也出现了。讨论了三龄幼虫唾液腺分泌物形成的复杂模式及其在变态过程中组成可能发生的变化。
