Arendt A, McDowell J H, Hargrave P A
Department of Ophthalmology, University of Florida, Gainesville 32610.
Pept Res. 1993 Nov-Dec;6(6):346-52.
Multipin solid-phase peptide synthesis is widely used for epitope mapping of monoclonal and polyclonal antibodies. However, neither the chemical yield nor the homogeneity of products currently match those of solid-phase synthesis of peptides on resins. In order to improve synthesis parameters, we have repeated the standard procedure and introduced modifications during synthesis of model heptapeptides and peptides from the sequence of rhodopsin and other proteins. Good incorporation of amino acids using the multipin peptide synthesis system can now be obtained in less synthesis time and with less costly reagents.
多针固相肽合成广泛用于单克隆抗体和多克隆抗体的表位作图。然而，目前产物的化学产率和均一性均无法与树脂上肽的固相合成相匹配。为了改善合成参数，我们重复了标准程序，并在模型七肽以及视紫红质和其他蛋白质序列的肽合成过程中引入了改进措施。现在，使用多针肽合成系统可以在更短的合成时间内，用成本更低的试剂实现氨基酸的良好掺入。
