Ortega-Corona B G, García-Bulnes G, Esparza-Avalos N, Ponce-Monter H, Valencia-Sánchez A, Villanueva-Tello T, Gallegos Cigarroa A
Unidad de Investigación Biomédica, IMSS, México, D.F.
Arch Med Res. 1993 Spring;24(1):27-31.
The effect of antiestrogen U23,469 administration in vivo on the concentration of dopamine, norepinephrine and epinephrine in the plasma, cerebral cortex and hypothalamus in ovariectomized rats was investigated. Rats were treated with estradiol benzoate, progesterone and U23,469 in different doses, s.c., daily for 6 days. Control group was injected with sesame oil. Catecholamines were estimated by radioenzymatic assay. Six days of U23,469, estradiol benzoate, progesterone or its combination altered the catecholamine levels compared to the control. Dopamine decreased in plasma with progesterone and U23,469. In the cerebral cortex, progesterone and U23,469 increased significantly and in the hypothalamus all the treatments produced a decrease of catecholamines. The levels of NE were reduced with estradiol benzoate, progesterone and U23,469; there was no significant difference in the norepinephrine levels after different treatments in the cerebral cortex, but the NE levels were significantly decreased in the hypothalamus. Epinephrine showed differences related to the treatment, as in plasma, as in cerebral cortex and hypothalamus. These results suggest that antiestrogen treatment compared with the estradiol benzoate or progesterone may affect the catecholamine levels of the central nervous system and plasma and support the idea that AE could have an indirect effect on the catecholaminergic system.
研究了抗雌激素U23,469体内给药对去卵巢大鼠血浆、大脑皮层和下丘脑多巴胺、去甲肾上腺素和肾上腺素浓度的影响。大鼠分别皮下注射不同剂量的苯甲酸雌二醇、孕酮和U23,469，每日1次，共6天。对照组注射芝麻油。采用放射酶法测定儿茶酚胺。与对照组相比，U23,469、苯甲酸雌二醇、孕酮或其组合给药6天改变了儿茶酚胺水平。孕酮和U23,469使血浆中多巴胺降低。在大脑皮层，孕酮和U23,469使其显著升高，而在下丘脑，所有处理均使儿茶酚胺降低。苯甲酸雌二醇、孕酮和U23,469均使去甲肾上腺素水平降低；不同处理后大脑皮层去甲肾上腺素水平无显著差异，但下丘脑去甲肾上腺素水平显著降低。肾上腺素在血浆、大脑皮层和下丘脑均表现出与处理相关的差异。这些结果表明，与苯甲酸雌二醇或孕酮相比，抗雌激素治疗可能影响中枢神经系统和血浆中的儿茶酚胺水平，并支持抗雌激素可能对儿茶酚胺能系统产生间接影响的观点。
