Uchida Yuki, Samejima Yuki, Kamijo Shotaro, Hosonuma Masahiro, Izumizaki Masahiko
Department of Physiology, Showa University School of Medicine, Shinagawaku, JPN.
Department of Orthopedic Surgery, Showa University Fujigaoka Hospital, Yokohama, JPN.
Cureus. 2024 Jul 29;16(7):e65706. doi: 10.7759/cureus.65706. eCollection 2024 Jul.
The TWIK-related potassium (TREK) channel subfamily, including TREK1 and TREK2, is a novel cold receptor. Ostruthin, a TREK1 and TREK2 agonist, is a component found in the plant and is traditionally used as an anticancer medicine in Vietnam, with its stems and roots treating various ailments. The female hormone progesterone (P4) influences body temperature in women; however, the effect of P4 on thermoregulation via TREK has not been examined. This study aims to investigate the effects of P4 on thermoregulatory responses in ostruthin-administered ovariectomized rats, which are animal models of human menopause.
Wistar rats were ovariectomized and implanted with silastic tubes with or without P4 (P4(+) and P4(-) groups). The TREK agonist or vehicle was injected intraperitoneally. Body temperature, locomotor activity, tail skin temperature, and thermoregulatory behavior (assessed by tail-hiding behavior) were continuously measured. Plasma concentrations of catecholamines, triiodothyronine, and thyroxine were also measured.
In both the P4(+) and P4(-) groups, the change in body temperature was greater among the rats administered the TREK agonist compared to the vehicle. No significant differences were observed between the groups in locomotor activity, tail skin temperature, or tail-hiding behavior. The dopamine concentration in the P4(+) group was lower than that in the P4(-) group.
Ostruthin, the TREK agonist, increases body temperature in ovariectomized rats; however, P4 may not affect these responses in ovariectomized rats.
TWIK相关钾通道（TREK）亚家族，包括TREK1和TREK2，是一种新型冷感受器。蛇床子素是一种TREK1和TREK2激动剂，是一种植物成分，在越南传统上用作抗癌药物，其茎和根可治疗多种疾病。女性激素孕酮（P4）会影响女性体温；然而，P4通过TREK对体温调节的影响尚未得到研究。本研究旨在探讨P4对给予蛇床子素的去卵巢大鼠体温调节反应的影响，这些大鼠是人类更年期的动物模型。
将Wistar大鼠去卵巢，并植入含或不含P4的硅橡胶管（P4（+）组和P4（-）组）。腹腔注射TREK激动剂或赋形剂。连续测量体温、运动活动、尾皮肤温度和体温调节行为（通过藏尾行为评估）。还测量了血浆儿茶酚胺、三碘甲状腺原氨酸和甲状腺素的浓度。
在P4（+）组和P4（-）组中，与赋形剂相比，给予TREK激动剂的大鼠体温变化更大。两组在运动活动、尾皮肤温度或藏尾行为方面未观察到显著差异。P4（+）组的多巴胺浓度低于P4（-）组。
TREK激动剂蛇床子素可使去卵巢大鼠体温升高；然而，P4可能不会影响去卵巢大鼠的这些反应。
