Pardon P, Marly J
Ann Rech Vet. 1976;7(4):297-305.
CD-I female mice were immunized with a subcutaneous injection of heat-inactivated Brucella melitensis (strain 53 H 38) incorporated into a water-in-oil emulsion. One month later, the effectiveness of this immunization was investigated by studying quantitatively in the spleen the fate of an intraperitoneal challenge inoculum of approximately I X 10(6) viable B. abortus strain 544. In unvaccinated mice, the number of viable challenge bacteria increased until about the 10th day, decreased and then remained at a nearly constant level. In animals vaccinated with a suitable dose of inactivated Brucella in adjuvant, the number of challenge organisms decreased on the first two days, then increased, but remained at a lower level than that found in control animals; the spleens of control animals reached higher weights than those of vaccinated ones. The effect of graduated doses of challenge on immunized mice was investigated: the splenic infection diminished sooner when the challenge dose was weaker. The findings are discussed in relation to the methods used to test the potency of Brucella vaccines.
将热灭活的羊布鲁氏菌（菌株53 H 38）混入油包水乳液中，皮下注射免疫CD-I雌性小鼠。一个月后，通过定量研究脾脏中约1×10(6) 个活流产布鲁氏菌菌株544腹腔攻击接种物的命运，来调查这种免疫的效果。在未接种疫苗的小鼠中，活的攻击细菌数量一直增加到大约第10天，然后减少，接着维持在一个几乎恒定的水平。在用合适剂量的佐剂灭活布鲁氏菌接种的动物中，攻击微生物数量在头两天减少，然后增加，但仍低于对照动物中的水平；对照动物的脾脏重量比接种疫苗的动物更高。研究了不同剂量的攻击对免疫小鼠的影响：当攻击剂量较小时，脾脏感染更快减轻。结合用于测试布鲁氏菌疫苗效力的方法对这些发现进行了讨论。
