Pardon P, Marly J
Ann Rech Vet. 1978;9(3):419-25.
Guinea pigs were inoculated subcutaneously into a hind footpad with Brucella abortus. Unimmunized guinea pigs had little capacity for barring brucella from spreading to their spleens: the inoculum dose needed to infect 50% of the spleens one month after inoculation was about 10 brucella. When the size of the challenge inoculum reached approximately 5 x 10(3) ID50 a previous immunization with killed or virulent brucella: --did not prevent the challenge bacteria from reaching the lymph nodes near the inoculation site, but reduced the level of infection in these, --barred or reduced the spreading of brucella throughout the remainder of the reticuloendothelial system. Immunization with a small number of virulent brucella was more effective than immunization with a higher number of killed bacteria to produce such modifications of the repartition of challenge brucella.
将流产布鲁氏菌皮下接种到豚鼠的后足垫。未免疫的豚鼠阻止布鲁氏菌扩散到脾脏的能力较弱：接种后一个月感染50%脾脏所需的接种剂量约为10个布鲁氏菌。当攻击接种物的大小达到约5×10³ ID50时，先前用灭活或有毒力的布鲁氏菌进行免疫：——不能阻止攻击细菌到达接种部位附近的淋巴结，但可降低这些淋巴结中的感染水平，——阻止或减少布鲁氏菌在网状内皮系统其余部位的扩散。用少量有毒力的布鲁氏菌进行免疫比用大量灭活细菌进行免疫在改变攻击布鲁氏菌的分布方面更有效。
