Triplett E L, Jovine R V, Govind N S, Roman S J, Chang S S, Prézelin B B
Department of Biological Sciences, University of California, Santa Barbara 93106.
Mol Mar Biol Biotechnol. 1993 Aug;2(4):246-54.
Characterizations are presented for RNA, 2 cDNA libraries, and 2 full-length cDNA sequences encoding for photosynthetic light-harvesting peridinin-chlorophyll a-protein (PCP) in the dinoflagellate Heterocapsa pygmaea. Subsequent analyses of the PCP system also indicate that (1) it is represented by multiple nuclear encoded genes, (2) a subset of mRNAs encoding for PCP apoproteins are regulated by growth irradiance, (3) PCP preproteins are larger than the mature apoproteins, and (4) PCP cDNA clones sequenced thus far contain a conserved region but are not identical. Results are discussed in the context of photoadaptation in dinoflagellates.
本文对来自微小异帽藻(Heterocapsa pygmaea)的RNA、2个cDNA文库以及2个编码光合捕光多甲藻叶绿素a蛋白(PCP)的全长cDNA序列进行了表征。随后对PCP系统的分析还表明:(1)它由多个核编码基因代表;(2)编码PCP脱辅基蛋白的mRNA子集受生长辐照度调节;(3)PCP前体蛋白比成熟脱辅基蛋白大;(4)迄今为止测序的PCP cDNA克隆包含一个保守区域,但并不相同。本文在甲藻光适应的背景下对结果进行了讨论。