共生甲藻中多甲藻素-叶绿素a蛋白复合体的特性分析
Characterization of the peridinin-chlorophyll a-protein complex in the dinoflagellate Symbiodinium.
作者信息
Jiang Jing, Zhang Hao, Kang Yisheng, Bina David, Lo Cynthia S, Blankenship Robert E
机构信息
Department of Energy, Environmental & Chemical Engineering, Washington University in St. Louis, St. Louis, MO 63130, USA.
出版信息
Biochim Biophys Acta. 2012 Jul;1817(7):983-9. doi: 10.1016/j.bbabio.2012.03.027. Epub 2012 Apr 4.
Abstract
The water-soluble peridinin-chlorophyll a-proteins (PCPs) are one of the major light harvesting complexes in photosynthetic dinoflagellates. PCP contains the carotenoid peridinin as its primary pigment. In this study, we identified and characterized the PCP protein and the PCP gene organization in Symbiodinium sp. CS-156. The protein molecular mass is 32.7kDa, revealing that the PCP is of the monomeric form. The intronless PCP genes are organized in tandem arrays. The PCP gene cassette is composed of 1095-bp coding regions and spacers in between. Despite the heterogeneity of PCP gene tandem repeats, we identified a single form of PCP, the sequence of which exactly matches the deduced sequence of PCP gene clone 7 (JQ395030) by LC-MS/MS analysis of tryptic digested PCP, revealing the mature PCP apoprotein is 312 amino acids in length. Pigment analysis showed a peridinin-to-Chl a ratio of 4. The peridinin-to-Chl a Q(y) energy transfer efficiency is 95% in this complex.
摘要
水溶性多甲藻素 - 叶绿素a蛋白（PCPs）是光合甲藻中主要的捕光复合体之一。PCP含有类胡萝卜素多甲藻素作为其主要色素。在本研究中，我们鉴定并表征了共生甲藻Symbiodinium sp. CS - 156中的PCP蛋白和PCP基因结构。该蛋白分子量为32.7kDa，表明PCP为单体形式。无内含子的PCP基因串联排列。PCP基因盒由1095bp的编码区和其间的间隔区组成。尽管PCP基因串联重复存在异质性，但我们鉴定出单一形式的PCP，通过对胰蛋白酶消化的PCP进行LC - MS/MS分析，其序列与PCP基因克隆7（JQ395030）的推导序列完全匹配，表明成熟的PCP脱辅基蛋白长度为312个氨基酸。色素分析显示多甲藻素与叶绿素a的比例为4。在该复合体中，多甲藻素与叶绿素a的Q(y)能量转移效率为95%。
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