Shifman M A
Center for Medical Informatics, Yale University School of Medicine, New Haven, CT 06510.
Comput Appl Biosci. 1993 Oct;9(5):511-5. doi: 10.1093/bioinformatics/9.5.511.
Pulse field gel electrophoresis mapping is an important technique for characterizing large segments of DNA and constructing long-range restriction maps. We have developed a tool, PFGE MAPPER, to aid in the construction of pulse field electrophoresis gel maps. This tool helps construct pulse field gel maps from single and double digest experiments visualized by hybridization with single copy probes. The program is written in Think C and runs on Macintosh computers. An intuitive interface allows the user to interactively modify fragment sizes or errors, select fragments for analysis and recalculate the maps. Maps can be printed or saved for later viewing. After constructing and saving several maps in a region, PFGE MAPPER can be used to refine and extend the overall map by merging individual maps. This tool should be useful for constructing long-range restriction maps of genomic DNA and yeast artificial chromosomes.
脉冲场凝胶电泳图谱分析是表征大片段DNA和构建长距离限制酶切图谱的一项重要技术。我们开发了一个工具，即PFGE MAPPER，以协助构建脉冲场电泳凝胶图谱。该工具可帮助从通过与单拷贝探针杂交可视化的单酶切和双酶切实验构建脉冲场凝胶图谱。该程序用Think C编写，在Macintosh计算机上运行。直观的界面允许用户交互式地修改片段大小或错误、选择片段进行分析并重新计算图谱。图谱可以打印或保存以供以后查看。在构建并保存某一区域的多个图谱后，PFGE MAPPER可用于通过合并单个图谱来完善和扩展整体图谱。该工具对于构建基因组DNA和酵母人工染色体的长距离限制酶切图谱应是有用的。
