Den Dunnen J T, van Ommen G J
Department of Human Genetics, Sylvius Laboratory, State University Leiden, Netherlands.
Appl Biochem Biotechnol. 1993 Jan-Feb;38(1-2):161-77. doi: 10.1007/BF02916419.
The term pulsed-field gel electrophoresis (PFGE) is used as an acronym to indicate any technique that resolves (large) DNA molecules by continuous reorientation. It bridges the resolution gap between cytogenetic methods (> 5 Mb) and DNA analysis (< 50 kb). Initially, PFGE was used to study the chromosomal content of unicellular eukaryotic organisms of interest to genetic research and population health. Later, PFGE was used to construct megabase maps of segments of the human genome. Successfull utilization of PFGE requires the availability of very high-molecular weight DNA. This article describes the modification of standard DNA protocols necessary to handle large DNA molecules, based on its encapsidation in agarose.
脉冲场凝胶电泳（PFGE）一词用作首字母缩写词，指代通过连续重新定向来分离（大）DNA分子的任何技术。它弥合了细胞遗传学方法（> 5 Mb）和DNA分析（< 50 kb）之间的分辨率差距。最初，PFGE用于研究遗传研究和群体健康感兴趣的单细胞真核生物的染色体含量。后来，PFGE被用于构建人类基因组片段的兆碱基图谱。成功利用PFGE需要有非常高分子量的DNA。本文描述了基于将大DNA分子包裹在琼脂糖中对处理大DNA分子所需的标准DNA方案进行的修改。
