Yu S
Department of Cardiovascular Surgery, Xijing Hospital, Fourth Military Medical University, Xian.
Zhonghua Yi Xue Za Zhi. 1993 Jul;73(7):416-9, 448.
Endothelial cells in the aortic walls of inbred SD rats were cultured in MEM culture medium containing 10% calf serum, and an anoxia-reperfusion injury model was established. The effect of anoxia-reperfusion injury on cultured endothelial cells was studied by measurements of membrane fluidity, intracellular Ca content, the release rate of 51Cr and the uptake rate of trypan-blue. The effect of fructose 1-6 diphosphate (FDP) and captopril (Cap) on cultured endothelial cells was also investigated. The findings indicated that the two drugs might protect cultured endothelial cells from anoxia-reperfusion injury. The mechanism of anoxia-reperfusion injury and the protective effect of the two drugs on cultured endothelial cells were briefly discussed.
将近交系SD大鼠主动脉壁内皮细胞培养于含10%小牛血清的MEM培养基中，建立缺氧-再灌注损伤模型。通过测量膜流动性、细胞内钙含量、51Cr释放率和台盼蓝摄取率，研究缺氧-再灌注损伤对培养的内皮细胞的影响。还研究了1,6-二磷酸果糖（FDP）和卡托普利（Cap）对培养的内皮细胞的影响。研究结果表明，这两种药物可能保护培养的内皮细胞免受缺氧-再灌注损伤。并简要讨论了缺氧-再灌注损伤的机制以及这两种药物对培养的内皮细胞的保护作用。
