Flügel C, Liebe S, Voorter C, Bloemendal H, Lütjen-Drecoll E
Department of Anatomy II, University of Erlangen/Nürnberg, Germany.
Curr Eye Res. 1993 Oct;12(10):871-6. doi: 10.3109/02713689309020393.
The presence and distribution of alpha B-crystallin in the anterior segment of human, monkey and bovine eyes was investigated immunocytochemically. In all three species the most intense staining was seen in the lens and in the nonpigmented and pigmented epithelial cells covering the tips of the pars plicata of the ciliary body. The staining intensity of the ciliary epithelial cells was comparable to that seen in the lens fibers. Strong labeling was also found in the corneal endothelium. In bovine eyes the presence of alpha B-crystallin in lens, ciliary epithelium of the pars plicata and corneal endothelium was also shown by biochemical analysis using SDS-PAGE and immunoblotting.
采用免疫细胞化学方法研究了αB-晶状体蛋白在人、猴和牛眼前节的存在及分布情况。在所有这三个物种中，在晶状体以及覆盖睫状体褶尖端的非色素和色素上皮细胞中观察到最强的染色。睫状体上皮细胞的染色强度与晶状体纤维中的染色强度相当。在角膜内皮中也发现了强标记。在牛眼中，通过SDS-PAGE和免疫印迹的生化分析也证实了αB-晶状体蛋白在晶状体、睫状体褶的睫状体上皮和角膜内皮中的存在。
