Ogueta S B, Solari A, Téllez-Iñón M T
Instituto de Investigaciones en Ingenieria Genética y Biologia Molecular (INGEBI) UBA, Argentina.
FEBS Lett. 1994 Jan 17;337(3):293-7. doi: 10.1016/0014-5793(94)80212-2.
Trypanosoma cruzi epimastigote forms showed a tightly bound Ca(2+)-calmodulin-dependent protein kinase activity, which could be partially extracted from membranes and axonemes. The enzyme is constituted by subunits which were autophosphorylated in the absence of exogenous substrates. An antibody against CaM kinase II recognized a Ca(2+)- or Ca(2+)-CaM-dependent conformational epitope in these fractions. The detected bands were of molecular weights similar to the alpha and beta subunits of the corresponding bovine brain enzyme (60 and 50 kDa). Studies using [125I]CaM revealed the presence of a CaM-binding domain. These experiments confirm that the parasite possesses a particulate CaM kinase with characteristics similar to the bovine brain enzyme.
克氏锥虫前鞭毛体形式显示出紧密结合的钙调蛋白依赖性蛋白激酶活性，该活性可部分从膜和轴丝中提取。该酶由在无外源底物时可自动磷酸化的亚基组成。针对钙调蛋白激酶II的抗体识别这些组分中一个钙或钙-钙调蛋白依赖性构象表位。检测到的条带分子量与相应牛脑酶的α和β亚基相似（60和50 kDa）。使用[125I]钙调蛋白的研究揭示了钙调蛋白结合域的存在。这些实验证实该寄生虫拥有一种颗粒状钙调蛋白激酶，其特性与牛脑酶相似。
