利用聚合物网络中的毛细管电泳检测DNA中的点突变。

Detection of point mutations in DNA using capillary electrophoresis in a polymer network.

作者信息

Kuypers A W, Willems P M, van der Schans M J, Linssen P C, Wessels H M, de Bruijn C H, Everaerts F M, Mensink E J

机构信息

Department of Internal Medicine, University Hospital Nijmegen, Netherlands.

出版信息

J Chromatogr. 1993 Nov 24;621(2):149-56. doi: 10.1016/0378-4347(93)80090-q.

DOI:10.1016/0378-4347(93)80090-q

PMID:8294536

Abstract

The use of capillary electrophoresis (CE) in a polymer network for single-strand conformation polymorphism (SSCP) is investigated. SSCP is a method to detect DNA point mutations, essential in the diagnosis of several diseases. The PCR (polymerase chain reaction) amplified p53 gene, a tumour suppressor gene known to be frequently mutated in malignant cells, was subjected to CE analysis. Two single-strand DNA fragments of 372 bp in length differing in only one nucleotide could be separated. We conclude that SSCP using CE in a polymer network is a powerful method for the detection of point mutations in DNA sequences.

摘要

研究了在聚合物网络中使用毛细管电泳（CE）进行单链构象多态性（SSCP）分析。SSCP是一种检测DNA点突变的方法，在多种疾病的诊断中至关重要。对经聚合酶链反应（PCR）扩增的p53基因（一种已知在恶性细胞中经常发生突变的肿瘤抑制基因）进行CE分析。两个长度为372 bp、仅一个核苷酸不同的单链DNA片段能够被分离。我们得出结论，在聚合物网络中使用CE的SSCP是检测DNA序列中点突变的有力方法。