Andresen A T, Rasmussen K E, Rugstad H E
Institute of Pharmacy, University of Oslo, Norway.
J Chromatogr. 1993 Nov 24;621(2):189-98. doi: 10.1016/0378-4347(93)80095-l.
Free phenytoin in human plasma was automatically determined by on-line equilibrium dialysis using the automated sequential trace enrichment of dialysate (ASTED) sample preparation system and HPLC. The dialysis cell was a modification of the cell supplied with the ASTED. Total phenytoin was analysed with the same analytical set-up and plasma protein binding was determined. Free phenytoin was determined in plasma from epileptic patients and the results were compared to those obtained by ultrafiltration. Automated determination of free and total phenytoin in plasma by the ASTED-HPLC combination was shown to be an accurate and reproducible method and the results in free phenytoin analyses were in agreement with those found with ultrafiltration. The sample throughput with the automated on-line combination of dialysis and column-switching HPLC was 75 samples in 24 h when the sample was dialysed at 37 degrees C.
使用透析液自动连续痕量富集（ASTED）样品制备系统和高效液相色谱法（HPLC），通过在线平衡透析自动测定人血浆中的游离苯妥英。透析池是对ASTED所提供透析池的一种改进。用相同的分析装置分析总苯妥英，并测定血浆蛋白结合率。测定了癫痫患者血浆中的游离苯妥英，并将结果与超滤法所得结果进行比较。结果表明，ASTED-HPLC联用自动测定血浆中游离和总苯妥英是一种准确且可重复的方法，游离苯妥英分析结果与超滤法结果一致。当样品在37℃下透析时，透析与柱切换HPLC的自动在线联用的样品通量为24小时75个样品。
