采用在线透析和高效液相色谱法对血浆中三种抗癫痫药物的游离浓度和总浓度进行自动化分析。

Automated analysis of free and total concentrations of three antiepileptic drugs in plasma with on-line dialysis and high-performance liquid chromatography.

作者信息

Johansen K, Krogh M, Andresen A T, Christophersen A S, Lehne G, Rasmussen K E

机构信息

Institute of Pharmacy, University of Oslo, Norway.

出版信息

J Chromatogr B Biomed Appl. 1995 Jul 21;669(2):281-8. doi: 10.1016/0378-4347(95)93203-4.

DOI:10.1016/0378-4347(95)93203-4

PMID:7581904

Abstract

A fully automated method for determination of the free and total concentration of drugs with a varying degree of protein binding is described. The antiepileptic drugs phenytoin, carbamazepine and phenobarbitone were chosen to demonstrate the utility of this technique. The method was based on the ASTED system and combined on-line equilibrium dialysis at 37 degrees C with concentration of the dialysate on a trace enrichment column and HPLC determination with UV detection. The dialysis cell was a modification of the ASTED dialysis cell and 22% of the free concentration of the drugs were recovered in the recipient channel of the dialyser after 10 min of dialysis at 37 degrees C. The free concentration, the total concentration as well as the drugs protein binding could be determined. The method was shown to be well suited for routine monitoring of the free and the total concentrations of the drugs in plasma from epileptic patients.

摘要

本文描述了一种全自动方法，用于测定具有不同程度蛋白质结合的药物的游离浓度和总浓度。选择抗癫痫药物苯妥英、卡马西平和苯巴比妥来证明该技术的实用性。该方法基于ASTED系统，将37℃下的在线平衡透析与在微量富集柱上对透析液进行浓缩以及采用紫外检测的高效液相色谱测定相结合。透析池是对ASTED透析池的一种改进，在37℃下透析10分钟后，22%的药物游离浓度在透析器的接受通道中被回收。可以测定游离浓度、总浓度以及药物的蛋白质结合情况。结果表明，该方法非常适合对癫痫患者血浆中药物的游离浓度和总浓度进行常规监测。