用于测定醛固酮排泄量的固相放射免疫分析法。

Solid phase radioimmunoassay for the determination of aldosterone excretion.

作者信息

Török A

机构信息

Department of Medical Chemistry, Albert Szent-Györgyi Medical University, Szeged, Hungary.

出版信息

J Immunoassay. 1993 Dec;14(4):297-306. doi: 10.1080/15321819308019856.

DOI:10.1080/15321819308019856

PMID:8294570

Abstract

A solid phase radioimmunoassay (SP-RIA) was developed for the determination of urinary conjugated aldosterone. Acid-hydrolyzed urine was extracted with dichloromethane and the extract was analyzed by SP-RIA. Microtiter plates were coated with two different anti-aldosterone antisera (S-3: against aldosterone-21-hemisuccinate-BSA and 58: aldosterone-3-(O-carboxymethyl)-oxime-BSA separately and preincubated with 3H-aldosterone. Reference values were obtained using a liquid radioimmunoassay method including paper chromatography. Regression analysis revealed for S-3: Y = 0.8 X + 3.2, r = 0.84; p < 0.0001; n = 36 and for 58: Y = 1.16 X + 1.2, r = 0.89; p < 0.0001; n = 36. The SP-RIA assay is useful for routine measurement of conjugated aldosterone excretion. The method is time-saving and does not require paper chromatography, and is adequate for many clinical purposes.

摘要

开发了一种固相放射免疫分析法（SP-RIA）用于测定尿中结合型醛固酮。酸水解后的尿液用二氯甲烷萃取，萃取物用SP-RIA分析。微量滴定板分别用两种不同的抗醛固酮抗血清包被（S-3：抗醛固酮-21-半琥珀酸-BSA和58：醛固酮-3-（O-羧甲基）-肟-BSA），并与³H-醛固酮预孵育。使用包括纸层析法的液体放射免疫分析法获得参考值。回归分析显示，对于S-3：Y = 0.8X + 3.2，r = 0.84；p < 0.0001；n = 36；对于58：Y = 1.16X + 1.2，r = 0.89；p < 0.0001；n = 36。SP-RIA分析法可用于常规测量结合型醛固酮排泄。该方法省时，不需要纸层析法，适用于多种临床目的。