Shen G X, Zhu H F, Zhang Y, Wang X L, Shao J F, Yang D F
Laboratory of Immunology, Research Center of Experimental Medicine, Tongji Medical University, Wuhan.
J Tongji Med Univ. 1993;13(3):134-7. doi: 10.1007/BF02886503.
The activity of interleukin 2 (IL-2) in culture supernatants of lymphokine-activated killer (LAK) cells and tumor infiltrating lymphocytes (TIL) as well as cytotoxicity of LAK cells on cultured leukemic cells were determined by MTT colorimetry. The results showed that higher activity of IL-2 in culture supernatant of LAK and TIL cells was found; it could be used to support the culture of IL-2 dependent cell lines. The significant cytotoxicity of LAK cells on leukemic cell lines could be found in vitro, and it was consistent with the ratio of effector cells to target cells. The number of living leukemic cells is consistently related with the concentration of formazan metabolite of MTT. It suggested that the numbers of living cells and cytotoxicity of LAK cells could be estimated by determination of formazan metabolite OD value.
采用MTT比色法测定了淋巴因子激活的杀伤细胞（LAK细胞）和肿瘤浸润淋巴细胞（TIL）培养上清中白细胞介素2（IL-2）的活性以及LAK细胞对培养白血病细胞的细胞毒性。结果显示，LAK细胞和TIL细胞培养上清中IL-2活性较高；可用于支持依赖IL-2的细胞系培养。体外可发现LAK细胞对白血病细胞系具有显著的细胞毒性，且与效应细胞与靶细胞的比例一致。存活白血病细胞数量与MTT甲臜代谢产物浓度始终相关。提示通过测定甲臜代谢产物OD值可估算LAK细胞的存活细胞数和细胞毒性。
