Nakahara K
Department of Clinical Pathology, Kyorin University School of Medicine, Mitaka.
Rinsho Byori. 1993 Dec;41(12):1296-304.
Leukemic cells as well as normal blood cells possess several antigens on their cell surfaces. By detecting these cell-type-specific molecules using monoclonal antibodies and flow cytometry, these immunological methods can be applied to both clinical and basic tumor studies. The analysis of cell surface markers has been widely applied in the diagnosis and the management of hematological disorders such as leukemias and lymphomas and it has become to be utilized as the routine work in many laboratories. Such analysis has been particularly useful in lymphocytic leukemias due to the ability of this method to classify lymphocyte subtypes, as well as specify their developmental origin. Importance is that, with this information, it is possible to determine expected clinical prognosis, to recommend treatment options and to judge treatment efficacy. In addition, two- or three-color staining for cell surface markers using fluorescein isothiocyanate (FITC)-, phycoerythrin (PE)- and/or peridinin chlorophyll protein (per CP)- conjugated monoclonal antibodies can provide further detailed and more precise analysis.
白血病细胞和正常血细胞在其细胞表面都拥有多种抗原。通过使用单克隆抗体和流式细胞术检测这些细胞类型特异性分子，这些免疫学方法可应用于临床和基础肿瘤研究。细胞表面标志物的分析已广泛应用于白血病和淋巴瘤等血液系统疾病的诊断和管理，并且已成为许多实验室的常规工作。由于该方法能够对淋巴细胞亚型进行分类并确定其发育起源，这种分析在淋巴细胞白血病中特别有用。重要的是，有了这些信息，就有可能确定预期的临床预后、推荐治疗方案并判断治疗效果。此外，使用异硫氰酸荧光素（FITC）、藻红蛋白（PE）和/或多甲藻叶绿素蛋白（PerCP）偶联的单克隆抗体对细胞表面标志物进行双色或三色染色，可以提供更详细、更精确的分析。
