Tasaka T, Nakahara K
Department of Clinical Pathology, Kyorin University School of Medicine.
Nihon Rinsho. 1992 Oct;50(10):2450-4.
With the popularity of flow cytometry (FCM) and the availability of a wide variety of monoclonal antibodies to human blood cells, analysis of cell surface antigens is now conducted at many facilities as part of their routine testing. Analysis of cell surface antigens by FCM has contributed greatly to the diagnosis and the determination of therapeutic effects in many diseases. In the present study, for detection of lymphocyte surface antigens by two color analysis and selection of appropriate antibodies are described. In addition, the significance of two color analysis is explained by citing hematological neoplasms and several other clinical cases. It has been recently reported that the immunological system is involved in many disease processes. In the future, more detailed studies will be conducted to elucidate the immunological mechanisms in these diseases.
随着流式细胞术（FCM）的普及以及针对人类血细胞的多种单克隆抗体的可得性，细胞表面抗原分析如今已在许多机构作为其常规检测的一部分进行。通过FCM进行细胞表面抗原分析在许多疾病的诊断和治疗效果判定方面发挥了巨大作用。在本研究中，描述了通过双色分析检测淋巴细胞表面抗原以及选择合适抗体的方法。此外，通过引用血液系统肿瘤和其他一些临床病例来解释双色分析的意义。最近有报道称免疫系统参与许多疾病过程。未来，将开展更详细的研究以阐明这些疾病中的免疫机制。
