Carraro S, Montiel M, Celener D, Pons S, Navigante A, Bustos D, Bustos Fernández L
Instituto de Gastroenterología Dr. Jorge Pérez Companc, Buenos Aires, Argentina.
Medicina (B Aires). 1993;53(2):133-6.
The role of short chain fatty acids (SCFA) in murine colonic carcinogenesis (MCC) has not yet been clarified. In rats, Freeman et al have reported an increased number of colonic tumors induced with dimethylhydrazine (DMH) and sodium butyrate in drinking water. On the other hand, Deschner et al showed that tributyrin intake did not increase MCC induced with azoxymethane. Both of them have reported high levels of fecal butyric acid with sodium butyrate and tributyrin intake. Although salt intake has been positively associated with colorectal cancer some authors do not support this association. We have evaluated the influence of right hemicolectomy (RH) (right colon as main source of SCFA) and the intake of 2%-pH 7 sodium butyrate (S.BUT) and 4 g/l sodium chloride (S.CHL) in drinking water, in MCC. Forty eight male Wistar rats weighing 150 g were divided into 4 groups: RH, S.BUT, S.CHL, control (C). Half of the animals received weekly DMH 20 mg/kg subcutaneously for 12 weeks. Necropsy was performed after 6 months. We have determined fecal SCFA content by gas chromatography. Neoplasm was present in 70% of rats treated with DMH. The number of animals with tumors was: RH 4/6, S.BUT 4/6, S.CHL 3/5, C 6/6. Tumor frequency was: RH 1.17 +/- 0.48, S.BUT 1.50 +/- 0.76, S.CHL 1.20 +/- 0.49, C 1.50 +/- 0.22. S.BUT group, treated with DMH, presented a lower butyric acid concentration (p < 0.05) in comparison with other groups. We have no explanation for this finding; gastric absorption of sodium butyrate may be an important factor.(ABSTRACT TRUNCATED AT 250 WORDS)
短链脂肪酸（SCFA）在小鼠结肠癌发生（MCC）中的作用尚未明确。在大鼠中，弗里曼等人报告称，饮用水中添加二甲基肼（DMH）和丁酸钠会导致结肠肿瘤数量增加。另一方面，德施纳等人表明，摄入三丁酸甘油酯不会增加由氧化偶氮甲烷诱导的MCC。他们两人都报告说，摄入丁酸钠和三丁酸甘油酯会使粪便中丁酸水平升高。尽管盐的摄入量与结直肠癌呈正相关，但一些作者并不支持这种关联。我们评估了右半结肠切除术（RH）（右结肠是SCFA的主要来源）以及饮用水中添加2% - pH 7的丁酸钠（S.BUT）和4 g/l氯化钠（S.CHL）对MCC的影响。48只体重150 g的雄性Wistar大鼠被分为4组：RH组、S.BUT组、S.CHL组、对照组（C）。一半的动物每周皮下注射20 mg/kg的DMH，共12周。6个月后进行尸检。我们通过气相色谱法测定了粪便中SCFA的含量。接受DMH治疗的大鼠中有70%出现了肿瘤。有肿瘤的动物数量分别为：RH组4/6、S.BUT组4/6、S.CHL组3/5、C组6/6。肿瘤发生率分别为：RH组1.17±0.48、S.BUT组1.50±0.76、S.CHL组1.20±0.49、C组1.50±0.22。接受DMH治疗的S.BUT组与其他组相比，丁酸浓度较低（p < 0.05）。我们无法解释这一发现；丁酸钠的胃吸收可能是一个重要因素。（摘要截断于250字）
