Wu J, He G, Wu G, Ruan C
Jiangsu Institute of Hematology, Thrombosis and Hemostasis Research Unit, Suzhou Medical College, China.
Nucl Med Commun. 1993 Dec;14(12):1088-92. doi: 10.1097/00006231-199312000-00007.
In canine experimental models of femoral arterial and venous thrombosis, the feasibility of thrombus imaging with 99Tcm-labelled monoclonal antibody SZ-51 to an alpha-granule membrane protein (GMP-140) on the surface of activated platelets was investigated. Both the arterial and venous thrombi were clearly discernible at 2 to 8 h after injection of 99Tcm-SZ-51. The suitable imaging time was 4 to 6 h. These results were confirmed by ex vivo images of thrombosed arteries and veins followed by killing the dogs at 8 h postinjection of 99Tcm-SZ-51. Quantitative imaging analysis showed that the ratios between the thrombus and the opposite vessel were increased strikingly over time. The ratios of thrombus to blood or surrounding muscle were 57.09 +/- 12.24 and 177.46 +/- 9.00 for arterial thrombi and 6.43 +/- 4.70 and 83.22 +/- 68.98 for venous thrombi at the time of death. Radiolabelled antibody was cleared from the blood with T1/2 alpha of 16.25 +/- 13.39 min and T1/2 beta of 7.28 +/- 1.15 h. These results show that it is feasible to detect noninvasively thrombus formation in vivo using 99Tcm-labelled monoclonal antibody SZ-51.
在犬股动脉和静脉血栓形成的实验模型中，研究了用99锝标记的抗活化血小板表面α-颗粒膜蛋白（GMP-140）单克隆抗体SZ-51进行血栓成像的可行性。注射99锝-SZ-51后2至8小时，动脉和静脉血栓均清晰可见。合适的成像时间为4至6小时。在注射99锝-SZ-51后8小时处死犬，对血栓形成的动脉和静脉进行体外成像，证实了这些结果。定量成像分析表明，血栓与对侧血管的比值随时间显著增加。死亡时，动脉血栓的血栓与血液或周围肌肉的比值分别为57.09±12.24和177.46±9.00，静脉血栓的比值分别为6.43±4.70和83.22±68.98。放射性标记抗体从血液中清除，α半衰期为16.25±13.39分钟，β半衰期为7.28±1.15小时。这些结果表明，使用99锝标记的单克隆抗体SZ-51在体内无创检测血栓形成是可行的。
