Wu G X, He G R, Wu J C, Ruan C G
Jiangsu Institute of Hematology, Suzhou Medical College.
Chin Med J (Engl). 1992 Jul;105(7):553-9.
To specifically detect the localization of thrombus in vivo, we prepared a monoclonal antibody (McAb) SZ-51 specific for an alpha-granule membrane protein (GMP-140) on the surface of activated human platelets. The thrombus binding rate in vitro was 80 +/- 5% for 131I-SZ-51 and 4.4 +/- 0.5% for 131I-nonimmune IgG. Owing to the crossreaction of McAb SZ-51 with the activated platelets of dogs, thrombus in the femoral artery and vein of dogs was formed and imaged with single photon emission computerized tomography (SPECT). The ratio of thrombus to blood radioactivity (T/B) was 2.1, 4.8, 14.0 and 18.0 at 1, 2, 3 and 4 hours after injection of 131I-SZ-51 (0.6 mCi, 50 micrograms) into the arterial thrombus respectively, while the T/B ratio was 1.7, 2.2, 5.3 and 8.0 for venous one. The injection of 131I-nonimmune IgG at the same doses yielded a T/B ratio of 1.2 +/- 0.3 at each time period for both arterial and venous thrombi. These findings were in good agreement with the counting of removed thrombi 24 hours after the injection of radiotracer. The ratio was 21.7 and 2.6 for the 131I-SZ-51 in the arterial and venous thrombus, respectively. However, there were only 1.48 and 1.6 for the 131I-nonimmune IgG. These results indicate the great potentiality of McAb SZ-51 in application to detection of thrombi in vivo.
为了在体内特异性检测血栓的定位，我们制备了一种针对活化人血小板表面α-颗粒膜蛋白（GMP-140）的单克隆抗体（McAb）SZ-51。131I-SZ-51的体外血栓结合率为80±5%，而131I-非免疫IgG的体外血栓结合率为4.4±0.5%。由于McAb SZ-51与犬活化血小板存在交叉反应，于是在犬的股动脉和静脉中形成血栓，并用单光子发射计算机断层扫描（SPECT）进行成像。向动脉血栓注射131I-SZ-51（0.6 mCi，50微克）后1、2、3和4小时，血栓与血液放射性比值（T/B）分别为2.1、4.8、14.0和18.0，而静脉血栓的T/B比值分别为1.7、2.2、5.3和8.0。注射相同剂量的131I-非免疫IgG后，动脉和静脉血栓在各时间段的T/B比值均为1.2±0.3。这些结果与注射放射性示踪剂24小时后取出的血栓计数结果高度一致。131I-SZ-51在动脉和静脉血栓中的比值分别为21.7和2.6。然而，131I-非免疫IgG在动脉和静脉血栓中的比值仅分别为1.48和1.6。这些结果表明McAb SZ-51在体内血栓检测应用中具有巨大潜力。
