McCulloch J, Williamson S A, Powis S J, Edington N
Department of Veterinary Pathology, Royal Veterinary College, London, UK.
Vet Microbiol. 1993 Oct;37(1-2):147-61. doi: 10.1016/0378-1135(93)90189-e.
It has been suggested that EHV-1 infection may perturb immune responsiveness in the natural equine host. The mechanism underlying this phenomenon is not clear, but disturbances of circulating leucocyte populations could contribute. In order to objectively assess the nature of the haematological changes provoked by EHV-1 infection, two groups of conventionally-maintained Welsh mountain ponies were challenge-infected intra-nasally with the Ab4 isolate of EHV-1. These groups were controlled by similarly-sized groups of non-infected ponies. All data generated was subjected to rigorous statistical analysis. Whole leucocyte count, neutrophil count, lymphocyte count, pan T cell count (RVC1 + cells-putative CD5 homologue), T cell subset count (RVC3 + cell-putative CD8 homologue), RVC2 + cells (putatively class II MHC+) and B cell count were recorded in experimental and control subjects at frequent intervals post-infection via flow cytometry. The principal abnormalities post-infection were T cell lymphopaenia, neutropaenia and the appearance of blastic cells of undetermined lineage. This study underlined the variability of EHV-1 infection in the natural, outbred equine host.
有人提出，EHV-1感染可能会扰乱天然马宿主的免疫反应。这种现象背后的机制尚不清楚，但循环白细胞群体的紊乱可能起了作用。为了客观评估EHV-1感染引起的血液学变化的性质，两组常规饲养的威尔士山地小马经鼻内接种EHV-1的Ab4分离株进行攻毒感染。这些组由同样大小的未感染小马组作为对照。对生成的所有数据进行了严格的统计分析。通过流式细胞术在感染后的不同时间间隔记录实验和对照动物的全白细胞计数、中性粒细胞计数、淋巴细胞计数、全T细胞计数（RVC1 +细胞 - 推定的CD5同源物）、T细胞亚群计数（RVC3 +细胞 - 推定的CD8同源物）、RVC2 +细胞（推定的II类MHC +）和B细胞计数。感染后的主要异常是T细胞淋巴细胞减少、中性粒细胞减少以及未确定谱系的母细胞出现。这项研究强调了EHV-1感染在天然、远交马宿主中的变异性。
