Kydd J H, Hannant D, Mumford J A
Centre for Preventive Medicine, Animal Health Trust, Newmarket, Suffolk, UK.
Vet Immunol Immunopathol. 1996 Jun 15;52(1-2):15-26. doi: 10.1016/0165-2427(95)05533-9.
This study characterised bronchoalveolar leucocytes collected by lavage from five susceptible and two immune ponies before and after nebulised aerosol infection with equid herpesvirus-1 (EHV-1). Leucocyte counts and lymphocyte phenotypic analyses were performed using either differential staining or an indirect immunofluorescence assay with monoclonal antibodies specific for equine (Eq) CD4, CD5, CD8 and B lymphocytes. After EHV-1 infection, significant changes developed: a transient neutrophilia occurred on Day 2, coincident with a reduction in macrophage numbers and an EqCD5+, EqCD4+ and EqCD8+ lymphopaenia. On Day 21, a significant rise in EqCD8+ lymphocytes with an associated fall in the EqCD4+:EqCD4+ ratio and fluctuations in B lymphocyte phenotypes were observed. This study shows that following EHV-1 infection, the bronchoalveolar compartment is subject to dynamic leucocyte migration which may have an important role in immunopathogenesis and recovery from disease.
本研究对5匹易感小马和2匹免疫小马在雾化吸入1型马疱疹病毒（EHV-1）感染前后通过灌洗收集的支气管肺泡白细胞进行了特征分析。使用鉴别染色或针对马（Eq）CD4、CD5、CD8和B淋巴细胞的单克隆抗体间接免疫荧光测定法进行白细胞计数和淋巴细胞表型分析。EHV-1感染后，出现了显著变化：第2天出现短暂的嗜中性粒细胞增多，同时巨噬细胞数量减少以及EqCD5 +、EqCD4 +和EqCD8 +淋巴细胞减少。在第21天，观察到EqCD8 +淋巴细胞显著增加，同时EqCD4 +:EqCD8 +比率下降以及B淋巴细胞表型波动。本研究表明，EHV-1感染后，支气管肺泡腔室会发生动态白细胞迁移，这可能在免疫发病机制和疾病恢复中起重要作用。
