Esnault V L, Short A K, Jones S J, Skehel M, Walker J, Lockwood C M
Service de Néphrologie-Immunologie Clinique, CHRU Nantes, France.
Adv Exp Med Biol. 1993;336:101-4. doi: 10.1007/978-1-4757-9182-2_16.
Thirty-five sera with binding greater than 20% in a myeloperoxidase (MPO, Calbiochem) ELISA were tested in Western blot analyses. 5/35 blotted MPO, but 5/35 blotted lactoferrin (LF) contaminating the commercial MPO preparation. All the anti-LF positive sera, but none of the anti-MPO positive sera, also exhibited anti-nuclear binding on Hep2 cells. Three of the patients with anti-LF antibodies had vasculitis affecting areas additional to the pulmonary-renal involvement which characterised the patients with anti-MPO antibodies.
