Flory D R, Miller L V, Fennessey P V
Department of Pharmacology, University of Colorado Health Sciences Center, Denver 80262.
Anal Chem. 1993 Dec 1;65(23):3501-4. doi: 10.1021/ac00071a029.
Stable isotopes have gained prominence in nutrition and trace element research. Fast atom bombardment mass spectrometry (FAB-MS) measurement of iron isotope ratios has an accuracy of > or equal to 99.7% with the stable isotopes (54)Fe, (56)Fe, and (58)Fe with relative standard deviations (RSDs) of < or equal to 0.9%. The isolation of iron from biological matrices can be accomplished in 4 h with almost total removal of isobaric interferences caused by (39)KOH, (39)K.H2O, (40)CaOH, and/or (40)Ca.H2O. FAB-MS isotope enrichment measurements from this method compare favorably to predicted absorption/enrichment levels.
稳定同位素在营养与微量元素研究中已变得日益重要。采用快原子轰击质谱法（FAB-MS）测量铁同位素比率时，对于稳定同位素（54）Fe、（56）Fe和（58）Fe，其准确度大于或等于99.7%，相对标准偏差（RSD）小于或等于0.9%。从生物基质中分离铁可在4小时内完成，几乎能完全去除由（39）KOH、（39）K.H2O、（40）CaOH和/或（40）Ca.H2O引起的同量异位干扰。此方法的FAB-MS同位素富集测量结果与预测的吸收/富集水平相比具有优势。
