Kawasaki N, Itoh N, Kawasaki T
College of Medical Technology, Kyoto University, Japan.
Biochem Biophys Res Commun. 1994 Jan 28;198(2):597-604. doi: 10.1006/bbrc.1994.1087.
Bovine conglutinin is a Ca(2+)-dependent serum lectin specific for N-acetylglucosamine. In the present study, we report the exon-intron organization and the sequence of 5'-regulatory region of the conglutinin gene. The coding region of conglutinin mRNA, which encoded the signal peptide and the sequence of the mature protein of 351 amino acids, consisted of seven exons. Exon III encoded the signal peptide, a cysteine-rich NH2-terminal region and six repeats of a Gly-X-Y triplet with one irregular triplet. The remaining long collagen-like domain (49 Gly-X-Y repeats with one irregular triplet) was encoded by four separate exons (Exon IV-VII). Exon VIII encoded the neck region and Exon IX encoded the carbohydrate-recognition domain. The overall exon-intron organization of conglutinin was very similar to that of mannan (mannose)-binding proteins. However, the presence of 108 bp exons in the gene encoding the collagen-like domain was a characteristic of conglutinin. In the promoter region of the gene, several putative consensus sequences that may be involved in the expression of conglutinin were identified.
牛胶固素是一种对N-乙酰葡糖胺具有特异性的Ca(2+)依赖性血清凝集素。在本研究中，我们报告了胶固素基因的外显子-内含子组织及5'-调控区序列。胶固素mRNA的编码区编码信号肽及由351个氨基酸组成的成熟蛋白序列，由7个外显子组成。外显子III编码信号肽、富含半胱氨酸的NH2-末端区域以及Gly-X-Y三联体的6个重复序列和1个不规则三联体。其余的长胶原样结构域（49个Gly-X-Y重复序列和1个不规则三联体）由4个独立的外显子（外显子IV-VII）编码。外显子VIII编码颈部区域，外显子IX编码碳水化合物识别结构域。胶固素的整体外显子-内含子组织与人甘露糖结合蛋白非常相似。然而，编码胶原样结构域的基因中存在108 bp外显子是胶固素的一个特征。在该基因的启动子区域，鉴定出了几个可能参与胶固素表达的推定共有序列。
