Lim B L, Holmskov U
Department of Zoology, University of Hong Kong, Pokfulam, Hong Kong.
Biochem Biophys Res Commun. 1996 Jan 5;218(1):260-6. doi: 10.1006/bbrc.1996.0046.
Bovine conglutinin is a collagenous C-type lectin (collectin) that is found in bovine serum. A recombinant protein, composed of the neck-region and the carbohydrate binding domain of bovine conglutinin, has been overexpressed in E. coli. The recombinant protein has been successfully renatured and showed the same sugar binding specificity as the native protein and was able to bind to yeast mannan and complement-activated immune complexes. The binding was calcium-dependent and was inhibited by N-acetylglucosamine. The concentration of N-acetylglucosamine required for 50% inhibition of binding to mannan was 1.77 mM for recombinant conglutinin and 0.71 mM for native conglutinin, respectively. The recombinant conglutinin should be useful in the assay and purification of circulating immune complexes and for therapeutic purposes involving the removal of immune complexes from patient's plasma.
牛凝集素是一种存在于牛血清中的胶原性C型凝集素（collectin）。一种由牛凝集素的颈部区域和碳水化合物结合结构域组成的重组蛋白已在大肠杆菌中过表达。该重组蛋白已成功复性，显示出与天然蛋白相同的糖结合特异性，并且能够结合酵母甘露聚糖和补体激活的免疫复合物。这种结合是钙依赖性的，并被N-乙酰葡糖胺抑制。对于重组凝集素和天然凝集素，分别需要1.77 mM和0.71 mM的N-乙酰葡糖胺才能50%抑制与甘露聚糖的结合。重组凝集素应可用于循环免疫复合物的检测和纯化，以及用于从患者血浆中清除免疫复合物的治疗目的。
