Vukicevic S, Latin V, Chen P, Batorsky R, Reddi A H, Sampath T K
Department of Anatomy, School of Medicine, Zagreb, Croatia.
Biochem Biophys Res Commun. 1994 Jan 28;198(2):693-700. doi: 10.1006/bbrc.1994.1100.
Osteogenic protein-1 (OP-1) is a member of the bone morphogenetic protein subfamily of the transforming growth factor-beta (TGF-beta) superfamily. Since members of the TGF-beta superfamily have a role in tissue development the distribution of OP-1 in developing human embryos (gestational age 5-14 weeks) was examined by immunohistochemical methods. Positive staining for OP-1 was observed in: sclerotome, hypertrophied chondrocytes, osteoblasts, periosteum, epithelial cells of the adrenal "provisional cortex" and the convoluted tubules of developing kidneys. In the developing lungs, pancreas and skin, OP-1 was localized in basement membranes underlying the epithelium. In vitro binding studies of 125I-OP-1 to various extracellular matrix components revealed high affinity of OP-1 for type IV collagen and less for heparin, collagen types I and VI. Present findings suggest that, in addition to bone formation, OP-1 could have other important regulatory roles in human embryogenesis with high binding affinity to a basement membrane component.
成骨蛋白-1（OP-1）是转化生长因子-β（TGF-β）超家族骨形态发生蛋白亚家族的成员。由于TGF-β超家族成员在组织发育中起作用，因此采用免疫组化方法检测了OP-1在发育中的人类胚胎（孕龄5-14周）中的分布。在以下部位观察到OP-1阳性染色：生骨节、肥大软骨细胞、成骨细胞、骨膜、肾上腺“临时皮质”的上皮细胞以及发育中肾脏的曲小管。在发育中的肺、胰腺和皮肤中，OP-1定位于上皮下方的基底膜。125I-OP-1与各种细胞外基质成分的体外结合研究表明，OP-1对IV型胶原具有高亲和力，对肝素、I型和VI型胶原的亲和力较低。目前的研究结果表明，除了骨形成外，OP-1在人类胚胎发生中可能还具有其他重要的调节作用，并且对基底膜成分具有高结合亲和力。
