Clemo F A, Crabtree W N, Walker E, DeNicola D B
Department of Veterinary Pathobiology, Purdue University, West Lafayette, Indiana 47907-1243.
Anal Quant Cytol Histol. 1993 Dec;15(6):418-26.
The DNA content of neoplasms is generally determined by flow cytometry, but quantitative microscopic image analysis is an alternative technique for DNA quantification. To compare these two methods, the DNA content of 31 paraffin-embedded canine transitional cell carcinomas was measured by flow cytometry and image analysis. Interpretable data were available for 26 samples by flow cytometry and 27 samples by image analysis. For 23 neoplasms with interpretable data by both methods, a good correlation (r = .80, P < .001) was obtained between the DNA indices determined by flow cytometry and image analysis. Concordance of DNA ploidy classification was reached in 21 samples; however, for 4 samples an aneuploid cell population, usually tetraploid, was identified by image analysis and not by flow cytometry. Two samples also had aneuploid peaks by flow cytometry and not by image analysis. Although a good correlation was seen between the measurement of DNA content of paraffin-embedded canine tissue by image analysis and flow cytometry, this study did demonstrate that aneuploid cell populations can be missed by both methods and that image analysis was better than flow cytometry in the detection of aneuploid cell populations, particularly tetraploid ones, in samples with excessive cellular debris.
肿瘤的DNA含量通常通过流式细胞术测定，但定量显微图像分析是一种用于DNA定量的替代技术。为比较这两种方法，通过流式细胞术和图像分析测量了31例石蜡包埋的犬移行细胞癌的DNA含量。流式细胞术可获得26个样本的可解释数据，图像分析可获得27个样本的可解释数据。对于两种方法均可获得可解释数据的23个肿瘤，流式细胞术和图像分析所测定的DNA指数之间具有良好的相关性（r = 0.80，P < 0.001）。21个样本的DNA倍体分类达成一致；然而，有4个样本通过图像分析鉴定出非整倍体细胞群，通常为四倍体，而流式细胞术未检测到。还有2个样本通过流式细胞术检测到非整倍体峰，而图像分析未检测到。尽管图像分析和流式细胞术对石蜡包埋犬组织DNA含量的测量之间具有良好的相关性，但本研究确实表明两种方法均可能遗漏非整倍体细胞群，并且在检测有过多细胞碎片的样本中的非整倍体细胞群（特别是四倍体细胞群）方面，图像分析优于流式细胞术。
