Goulandris N, Karakitsos P, Georgoulakis J, Bellos C, Deliveliotis C, Legaki S
Department of Urology, University of Athens, Greece.
J Urol. 1996 Sep;156(3):958-60. doi: 10.1016/s0022-5347(01)65671-4.
We compared the results of deoxyribonucleic acid (DNA) ploidy determinations performed by flow cytometry and image cytometry in transitional cell carcinomas of the bladder.
In 81 cases of transitional cell carcinoma of the bladder DNA indexes were measured by flow cytometry of bladder washings and tumor tissue samples, and by image cytometry of imprints from tumor tissue samples.
There was good correlation between bladder washings and tissue samples analyzed by flow cytometry in 68 cases but aneuploidy was missed with bladder washings in 13 (16%). There was also good correlation between flow and image cytometry in 75 cases (92.59%) regarding the detection of aneuploidy. There was agreement between detection of aneuploidy and DNA index in 45 cases (55.5%), while both methods detected an aneuploid population in 30 (37%) but there was disagreement regarding DNA index. Aneuploid populations were missed by flow cytometry in 6 cases (7.4%). Furthermore, in 10 cases peridiploid peaks were found on the image cytometry histograms, which were not visible on flow cytometry. However, it was not possible to assess accurately if these were true peridiploid populations.
There is good overall correlation between DNA content measured by flow and image cytometry but image cytometry has the advantage of visual discrimination, permitting preferential selection and analysis of tumor cells. However, certain problems remain with image cytometry, particularly in the case of peridiploid peaks, which cannot be classified accurately as showing true peridiploid or right shifted diploid populations.
我们比较了流式细胞术和图像细胞术测定膀胱移行细胞癌中脱氧核糖核酸（DNA）倍体的结果。
对81例膀胱移行细胞癌患者，通过膀胱冲洗液和肿瘤组织样本的流式细胞术以及肿瘤组织样本印片的图像细胞术来测量DNA指数。
68例中，膀胱冲洗液与流式细胞术分析的组织样本之间具有良好的相关性，但13例（16%）的膀胱冲洗液漏检了非整倍体。在非整倍体检测方面，流式细胞术与图像细胞术在75例（92.59%）中也具有良好的相关性。45例（55.5%）中非整倍体检测与DNA指数检测结果一致，两种方法均检测到非整倍体群体的有30例（37%），但在DNA指数方面存在分歧。流式细胞术漏检非整倍体群体6例（7.4%）。此外，在10例中，图像细胞术直方图上发现了亚二倍体峰，而在流式细胞术上不可见。然而，无法准确评估这些是否为真正的亚二倍体群体。
流式细胞术和图像细胞术所测DNA含量总体相关性良好，但图像细胞术具有视觉辨别优势，可优先选择和分析肿瘤细胞。然而，图像细胞术仍存在某些问题，特别是在亚二倍体峰的情况下，无法准确分类为真正的亚二倍体或右移二倍体群体。
