Ploczeková C, Földes O
Ustav preventívnej a klinickej medicíny, Bratislava.
Cesk Epidemiol Mikrobiol Imunol. 1992 Dec;41(6):346-54.
The author describes the use of polyclonal antibodies against gentamicin by competitive ELISA. The author used polystyrene microplates as the solid phase for antibodies. Gentamicin was marked by horse radish peroxidase and a known amount of thus labelled gentamicin competed for a bond with an antibody with an unknown amount of gentamicin in serum. The method requires 10 microliters non-diluted serum and the results can be evaluated within one hour. The accuracy of the method according to values of the coefficient of variation (CV) is 9.0-13.2% in different tests and 5.2 to 9.2% within one examination.
作者描述了通过竞争酶联免疫吸附测定法使用抗庆大霉素的多克隆抗体。作者使用聚苯乙烯微孔板作为抗体的固相。庆大霉素用辣根过氧化物酶标记，已知量的这种标记庆大霉素与血清中未知量的庆大霉素竞争与抗体结合。该方法需要10微升未稀释的血清，结果可在一小时内评估。根据变异系数（CV）值，该方法在不同测试中的准确度为9.0 - 13.2%，在一次检测内为5.2至9.2%。
