Holmes A S, Suhling K, Birch D J
Department of Physics and Applied Physics, University of Strathclyde, Glasgow, UK.
Biophys Chem. 1993 Dec;48(2):193-204. doi: 10.1016/0301-4622(93)85010-f.
Fluorescence quenching of perylene by Co2+ and Ni2+ ions has been investigated both below and above the phase transition temperature in small unilamellar DPPC vesicles. Classic Förster type energy transfer was observed for perylene quenched by Co2+ ions below the phase transition when the effects of donor-donor energy transfer are taken into account. In the liquid crystalline phase a simple diffusion theory incorporating energy transfer was found to model the system well. For quenching by Ni2+ ions both above and below the phase transition temperature in lipid bilayers and in glycerol the data did not follow classic Förster type energy transfer but indicated that an additional quenching mechanism was present. A mechanism other than Förster behaviour was also observed for the quenching by Cr3+ ions in glycerol.
在小单层二棕榈酰磷脂酰胆碱（DPPC）囊泡中，研究了低于和高于相变温度时，Co2+和Ni2+离子对苝的荧光猝灭作用。当考虑供体-供体能量转移的影响时，在相变温度以下，观察到Co2+离子猝灭苝的经典Förster型能量转移。在液晶相中，发现一个结合能量转移的简单扩散理论能很好地模拟该系统。对于脂质双层和甘油中高于和低于相变温度时Ni2+离子的猝灭作用，数据并不遵循经典的Förster型能量转移，而是表明存在额外的猝灭机制。在甘油中Cr3+离子的猝灭作用也观察到了不同于Förster行为的机制。
