Velazquez E, Ruiz-Albusac J M, Carrion M, Blazquez E
Departamento de Bioquímica y Biología Molecular, Facultad de Medicina, Universidad Complutense, Spain.
Neuroreport. 1993 Dec 13;5(3):261-4. doi: 10.1097/00001756-199312000-00020.
Brain lipids were labelled with [1-14C]-isethionyl acetimidate and purified by sequential thin layer chromatography. Four labelled peaks were obtained, the first ones migrating with the same Rf as glycosyl-phosphatidylinositol (GPI). Further proof of the isolation of GPI was obtained by the observations that 44.8% of the radioactivity associated with the lipid in peak I was converted to the water phase by the effect of a PI-specific phospholipase C, and that the soluble material so obtained produced a dose-dependent inhibition of cAMP-dependent protein kinase activity. These findings indicate a biological equivalence between GPI and its polar head group from rat brain and those described in other cell types, and are consistent with the proposed role of these molecules in cellular signalling.
脑脂质用[1-¹⁴C]-羟乙磺酰乙脒标记，并通过连续薄层层析法进行纯化。得到了四个标记峰，第一个峰的迁移率与糖基磷脂酰肌醇（GPI）相同。通过以下观察结果进一步证明了GPI的分离：与峰I中脂质相关的44.8%的放射性通过PI特异性磷脂酶C的作用转化到水相中，并且如此获得的可溶性物质产生了对cAMP依赖性蛋白激酶活性的剂量依赖性抑制。这些发现表明大鼠脑中的GPI及其极性头部基团与其他细胞类型中描述的具有生物学等效性，并且与这些分子在细胞信号传导中所提出的作用一致。
