Deckner M L, Frisén J, Verge V M, Hökfelt T, Risling M
Department of Neuroscience, Karolinska Institutet, Stockholm, Sweden.
Neuroreport. 1993 Dec 13;5(3):301-4. doi: 10.1097/00001756-199312000-00030.
We used in situ hybridization to localize trk, trkB and trkC mRNA, in rat and cat olfactory bulb. Expression of mRNA encoding truncated trkB receptors was seen in all layers, while only very modest full-length trkB expression could be detected. trkC hybridization was seen in all layers, most dense in the mitral cell layer. The localization of full-length tyrosine kinase trkB receptor in olfactory bulb and epithelium was examined with immunohistochemistry. trkB-like immunoreactivity was seen in the fila olfactoria, epithelium and in vitro, in olfactory sensory neurones. Since BDNF is expressed by olfactory sensory neurone target cells in the olfactory bulb, these data suggest that BDNF may act as a target derived neurotrophic factor in the primary olfactory system.
我们运用原位杂交技术在大鼠和猫的嗅球中定位trk、trkB和trkC mRNA。在所有层中均可见到编码截短型trkB受体的mRNA的表达，而仅能检测到非常少量的全长trkB表达。在所有层中均可见到trkC杂交信号，在二尖瓣细胞层最为密集。运用免疫组织化学方法检测了全长酪氨酸激酶trkB受体在嗅球和上皮中的定位。在嗅丝、上皮以及体外培养的嗅觉感觉神经元中可见到trkB样免疫反应性。由于BDNF由嗅球中嗅觉感觉神经元的靶细胞表达，这些数据提示BDNF可能作为初级嗅觉系统中一种靶源性神经营养因子发挥作用。
