Dragunow M, Lawlor P, Chiasson B, Robertson H
Department of Pharmacology, School of Medicine, University of Auckland, New Zealand.
Neuroreport. 1993 Dec 13;5(3):305-6. doi: 10.1097/00001756-199312000-00031.
Sodium pentobarbital anaesthetized rats were injected ipsilaterally with an antisense oligonucleotide to c-fos and contralaterally with a sense of oligonucleotide to c-fos in the striatum. Ten hours later they were injected with amphetamine or apomorphine and their behaviour was observed for a further 2 h at which time they were overdosed with anaesthetic and their brains were removed, frozen and processed for Fos and Jun B immunohistochemistry. Rats showed amphetamine- and apomorphine-induced rotation towards the antisense injected striatum. The antisense oligonucleotide also strongly inhibited the amphetamine-induced expression of c-Fos and Jun B in striatal neurones. These results suggest that antisense to c-fos produces a biochemical change in the injected striatum that then, 10 h later, blocks amphetamine- and apomorphine-induced behavioural and biochemical effects.
用戊巴比妥钠麻醉大鼠，在其纹状体同侧注射针对c-fos的反义寡核苷酸，对侧注射针对c-fos的正义寡核苷酸。10小时后，给它们注射苯丙胺或阿扑吗啡，并在接下来的2小时内观察其行为，之后过量注射麻醉剂，取出大脑，冷冻并进行Fos和Jun B免疫组织化学处理。大鼠表现出苯丙胺和阿扑吗啡诱导的向注射反义寡核苷酸的纹状体旋转。反义寡核苷酸还强烈抑制苯丙胺诱导的纹状体神经元中c-Fos和Jun B的表达。这些结果表明，针对c-fos的反义寡核苷酸在注射的纹状体中产生生化变化，然后在10小时后阻断苯丙胺和阿扑吗啡诱导的行为和生化效应。
