Sommer W, Bjelke B, Ganten D, Fuxe K
Department of Histology and Neurobiology, Karolinska Institutet, Stockholm, Sweden.
Neuroreport. 1993 Dec 13;5(3):277-80. doi: 10.1097/00001756-199312000-00024.
The immediate-early genes, especially c-fos, have been suggested to have an important role in neuronal plasticity and memory. However, it has been difficult to determine if in fact c-fos can participate in control of behaviour. Here we can report that direct unilateral infusion into the rat neostriatum of an antisense phosphothioate oligodeoxynucleotide to c-fos mRNA leads to the rapid induction of ipsilateral rotational behaviour after d-amphetamine administration. This is associated with an elimination of the in vivo expression of Fos-like immunoreactivity within the infused region of neostriatum. Following infusion of these oligodeoxynucleotides labelled with fluorescein isothiocyanate they appear with a nuclear and cytoplasmic location in a large number of nerve cells, many of which are immunoreactive for dopamine and cyclic AMP regulated phosphoprotein. The present findings suggest that the immediate-early gene c-fos has a role also in control of behavioural activity, as illustrated in D1 receptor containing striatal neurones.
即刻早期基因，尤其是c-fos，被认为在神经元可塑性和记忆中起重要作用。然而，很难确定c-fos是否真的能参与行为控制。在此我们可以报告，将一种针对c-fos mRNA的反义硫代磷酸酯寡脱氧核苷酸直接单侧注入大鼠新纹状体，会导致在给予d-苯丙胺后快速诱导同侧旋转行为。这与新纹状体注入区域内Fos样免疫反应性的体内表达消除有关。在用异硫氰酸荧光素标记这些寡脱氧核苷酸后注入，它们出现在大量神经细胞的细胞核和细胞质中，其中许多对多巴胺和环磷酸腺苷调节磷蛋白具有免疫反应性。目前的研究结果表明，即刻早期基因c-fos在行为活动控制中也起作用，如在含有D1受体的纹状体神经元中所示。
