Skalka A, Shapiro L
Gene. 1976;1(1):65-79. doi: 10.1016/0378-1119(76)90007-x.
A series of simple, in situ immunoassays have been developed which can be used in screening for translation products of genes cloned in vitro recombination experiments with either phage or plasmid vectors. Antigen-antibody complex formation occurring within a vector-phage plaque can be used to detect the production of a specific protein from an amplified gene. Immunoassays of colonies lysed in situ either by lambda prophage induction or by biochemical means afford a much higher level of sensitivity than the plaque assay probably adequate to detect the production of a few molecules of protein per cell.
已经开发出一系列简单的原位免疫测定法，可用于筛选在用噬菌体或质粒载体进行的体外重组实验中克隆的基因的翻译产物。载体 - 噬菌体噬菌斑内发生的抗原 - 抗体复合物形成可用于检测扩增基因产生的特定蛋白质。通过λ原噬菌体诱导或生化方法原位裂解菌落的免疫测定法比噬菌斑测定法具有更高的灵敏度，可能足以检测每个细胞中少量蛋白质分子的产生。
